Correlation of Single Nucleotide Polymorphism 35-Kb Upstream of Hla -C and Clinical Profile of Multidrug-Resistant Tuberculosis
Published: September 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.6451
Marwoto, Umi Hani’ Vismayanti Lismana, Afiono Agung Prasetyo, Suradi, Reviono, Harsini
1. A-Infection Genomic Immunology Cancer (A-IGIC) Research Group, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;
Department of Microbiology Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia.
2. A-Infection Genomic Immunology Cancer (A-IGIC) Research Group, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia.
3. A-Infection Genomic Immunology Cancer (A-IGIC) Research Group, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;
Department of Microbiology Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;
Center of Biotechnology and Biodiversity Research and Development, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia.
4. Department of Pulmonology Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia.
Correspondence
Dr. Afiono Agung Prasetyo,
A-IGIC (A-Infection, Genomic, Immunology & Cancer) Research Group,Center of Biotechnology and Biodiversity
Research and Development, Department of Microbiology, Faculty of Medicine, Sebelas Maret University,
Jl. Ir. Sutami 36A, Surakarta, Indonesia.
E-mail : afie.agp.la@gmail.com, afie@staff.uns.ac.id, afieagp@yahoo.com
Introduction: The SNP HLA-C-35 kb (rs9264942) may contribute to the host immune defense mechanism by affecting the cell surface expression pattern of HLA-C and antigen presentation to CD8+ cytotoxic cells. Thus, this SNP may contribute to intracellular multidrug-resistant (MDR)-tuberculosis (TB) infection.
Aim: To examine the association between the SNP HLA-C-35 kb (rs9264942) and the clinical profile of MDR-TB infection. Settings and Design: MDR-TB-positive patients were followed from May 2012 to December 2013 to observe the progression of MDR-TB infection. Non-TB individuals and non-MDR-TB individuals were also recruited as controls. Materials and Methods: The patients’ HLA-C-35 kb (rs9264942) status was determined by PCR.
Results: The C allele was slightly more frequent in the MDR-TB patients than in the non-MDR TB patients (OR= 1.28; 95% CI: 0.701 – 2.328). The C allele was found to be more frequent in the MDR-TB patients exhibiting pulmonary fibrosis (OR= 2.13; 95% CI: 0.606 – 7.480) or pulmonary infiltrates (OR= 3.17; 95% CI: 0.690 – 14.598) and among the MDR-TB patients who were classified as underweight (OR= 8.00; 95% CI: 1.261 – 50.770). The CC genotype was associated with the treatment after failure of category II group (OR= 4.17; 95% CI: 1.301 – 13.346).
Conclusion: The C allele SNP HLA-C-35 kb (rs9264942) may contribute to the clinical profile in MDR-TB infection.
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